Research

Ongoing Clinical Trials

1. A Safety, Tolerability, and Efficacy Study of VC-01™ Combination Product in Subjects With Type I Diabetes Mellitus [click here for study description]

   1. An Open-Label, First-In-Human, Study Evaluating the Safety, Tolerability, and Efficacy of VC-02™ Combination Product in Subjects with Type 1 Diabetes Mellitus and Hypoglycemia Unawareness [click here for study description]

2. Stem Cell Mobilization (Plerixafor) and Immunologic Reset in New Onset Type 1 Diabetes Mellitus [click here for study description]

3. Polyclonal Regulatory T cell (PolyTreg) Immunotherapy in Islet Transplantation [click here for study description]

Volunteer 

If you wish to register your interest to volunteer for one of our current or future research studies, please click here.

Publications

2018

1. Imamura H, Adachi T, Kin T, Ono S, Sakai Y, Adachi T, Soyama A, Hidaka M, Takatsuki M, Shapiro AMJ, Eguchi S. An engineered cell sheet composed of human islets and human fibroblast, bone marrow-derived mesenchymal stem cells, or adipose-derived mesenchymal stem cells: An in vitro comparison study. Islets 2018;1-11. 

2. Shapiro AMJ. Gearing up for stem cell-derived betal cells – are we ready? Transplantation 2018: doi: 10.1097/TP.0000000000002214 (e-published ahead of print).

3. Jones B, Buenaventura T, Kanda N, Chabosseau P, Owen BM, Scott R, Goldin R, Angkathunyakul N, Correa IR Jr, Bosco D, Johnson PR, Piemonti L, Marchetti P, Shapiro AMJ, Cochran BJ, Hanyaloglu AC, Inoue A, Tan T, Rutter GA, Tomas A, Bloom SR. Targeting GLP-1 receptor trafficking to improve agonist efficacy. Nat Commun 2018;9(1):1602.

4. Teo AKK, Lim CS, Cheow LF, Kin T, Shapiro JA, Kang NY, Burkholder W, Lau HH. Single-cell analyses of human islet cells reveal de-differentiation signatures. Cell Death Discov 2018; 4:14. Doi: 1038/S41420-017-0014-5.

5. Bruni A, Pepper AR, Pawlick RL, Gala-Lopez B, Gamble A, Kin T, Malcom AJ, Jones C, Piganelli JD, Crapo JD, Shapiro AMJ. BMX-001, a novel redox-active metalloporphyrin, improves islet function and engraftment in a murine transplant model. Am J Transplant 2018. Doi: 10.1111/ajt.14705 (e-published ahead of print).

6. Gamble A, Pepper AR, Bruni A, Shapiro AMJ. The journey of islet cell transplantation and future development. Islets 2018. Doi: 10.1080/19382014 (e-published ahead of print). 

7. Wallner K, Pedroza RG, Awotwe I, Piret JM, Senior PA, Shapiro AMJ,  McCabe C. Stem cells and beta cell replacement therapy: a prospective health technology assessment study. BMC Endocr Disrd 2018;18(1):6

8. Gala-Lopez BL, Neiman D, Kin T, O’Gorman D, Pepper AR, Malcolm AJ, Pianzin S, Senior PA, Campbell P, Glaser B, Dor Y, Shemer R. Shapiro AMJ. Beta cell death by cell-free DNA and outcome after clinical islet transplantation. Transplantation 2018. Doi: 1097/TP.0000000000002083. (e-published ahead of print.

9. Sabatini PV, Speckmann T, Nian C, Glavas MM, Wong CK, Yoon JS, Kin T, Shapiro AMJ, Gibson WT, Verchere CB, Lynn FC. Neuronal PAS domain protein F suppression of oxygen sensing optimizes metabolism during excitation of neuroendocrine cells. Cell Rep 2018;22(1):163-174.

2017

1. Bruni A, McCall M, Shapiro AMJ. Chapter 13: Islet Cell Transplantation. Ledbetter DJ, Johnson PV, Editors. Endocrine Surgery in Children. Springer 2017.

2. Koh A, Senior PE, Shapiro AMJ. Chapter 69: Islet Transplantation. Holt R, Cockram C, Flyvbjerg A, Goldstein B, Editors. Wiley 2017

3. Martinez-Sanchez A, Nguyen-Tu MS, Cebola I, Yavari A, Marchetti P, Piemonti L, de Koning E, Shapiro AMJ, Johnson P, Sakamoto K, Smith DM, Leclerc I, Ashrafian H, Ferrer J, Rutter GA.  MiR-184 expresssion is regulated by AMPK in pancreatic islets.  FASEB J 2017.  Doi: 10.1096/fj.201701100R.  

4. Buenaventura T, Kanda N, Douzenis PC, Jones B, Bloom SR, Chabosseau P, Correa IR Jr, Bosco D, Piemonti L, Marchetti P, Johnson PR, Shapiro AJ, Rutter GA, Tomas A.  A targeted RNAi screen identifies endocytic trafficking factors that control GLP-1 receptor signaling in pancreatic beta cells.  Diabetes 2017. Doi: 10.2337/db17-0639.

5. Neiman D, Moss J, Hecht M, Magenheim J, Piyanzin S, Shapiro AMJ, de Koning EJP, Razin A, Cedar H, Shemer R, Dor Y.  Islet cells share promoter hypomethylation independently of expression, but exhibit cell-type-specific methylation in enhancers.  Proc Natl Acad Sci USA 2017; pii: 201712736.  Doi:  10.1073/pnas.1713736114.

6. Ling Y, Gill A, Oram R, Shapiro AMJ, Senior P.  Clinical islet transplanatation for type 1 diabetes in Canada:  Referral patterns and eligibility assessment.  Can J Diabetes 2017 pii: S1499-2671(17)30306-4. doi: 10.1016/j.jcjd.2017.10.039. 

7. Pawlick R, Gala-Lopez B, Pepper AR, Abualhassan N, Bruni A, Suzuki K, Rayat G, Elliott JF, Shapiro AMJ.  Low energy x-ray (grenz ray) treatment of purified islets prior to allotransplant markedly decreases passenger leukocyte populations.  Islets 2017;9(4):e1330742 doi:  10.1080/19382014. 

8. Imamura H, Eguchi S, Shapiro AMJ, Kin T.  A case of double common bile duct in a deceased donor for transplantation.  Surg Radiol Anat 2017.  Doi: 10.1007/s00276-017-1874-3.

9. Pepper AR, Pawlich R, Bruni A, Wink J, Rafiei Y, O’Gorman D, Yan-Do R, Gala-Lopez B, Kin T, MacDonald PE, Shapiro AMJ.  Transplantation of human pancreatic endoderm cells reverses diabetes post transplantation in a prevascularized subcutaneous site.  Stem Cell Reports 2017;8(6):1689-1700.

10. Peters A, Olateju T, Deschenes J, Shankarnarayan SH, Chua N, Shapiro AM, Senior P.  Post-transplant lymphoproliferative disorder following clinical islet transplantation:  Report of the first two cases.  Am J Transplant 2017.  Doi:  10.1111/ajt.14303.

11. Shapiro AM.  Response to “normothermic machine perfusion:  A new world deserving careful exploration. Am J Transplant 2017.  Doi:  10.1111/ajt.14281.

12. Pepper AR, Bruni A, Pawlick R, Wink J, Rafiei Y, Gala-Lopez B, Bral M, Abualhassan N, Kin T, Shapiro AMJ.  Engraftment site and effectiveness of the pan-caspase inhibitor F573 to improve engraftment in mouse and human islet transplantation in mice.  Transplantation 2017:doi:  1097/TP.0000000000001638.  

13. Senior PA, Koh A, Yau J, Imes S, Dinyari P, Malcolm AJ, Light P, Shapiro AM.  Sitagliptin plus pantoprazole can restore but not maintain insulin independence after clinical islet transplantation:  results of a pilot study.  Diabet Med 2017;34(2):204-212.

2016

1. Anderson B, Nostedt J, Girgis S, Dixon T, Agrawal V, Wiebe E, Senior PA, Shapiro AM.  Insulinoma or non-insulinoma pancreatogenous hypoglycemia?  A diagnostic dilemma.  J Surg Case Rep 2016:2016(11) pii: rjw188. doi:  10.1093/jscr/rjw188 

2. Shapiro AM, Pokryczynska M, Ricordi C.  Clinical pancreatic islet transplantation.  Nat Rev Endocrinol 2017; 13(5):268-277

3. Gala-Lopez BL, Pepper AR, Dinyari P, Malcolm AJ, Kin T, Pawlick R, Senior PA, Shapiro AMJ.  Subcutaneous clinical islet transplantation in a prevascularized subcutaneous pouch – preliminary experience.  CellR4 2016;4(5): E2132.

4. Pepper AR, Bruni A, Pawlick RL, Gala-Lopez B, Rafiei Y, Wink J, Kin T, Shapiro AM.  Long-term function and optimization of mouse and human islet transplantation in the subcutaneous device-less site.  Islets 2016; 8(6):186-194 

5. Bral M, Gala-Lopez B, Bigam D, Kneteman N, Malcolm A, Livingstone S, Anres A, Emamaullee J, Russell L, Coussios C, West LJ, Friend PJ, Shapiro AM.  Preliminary single centre Canadian experience of human normothermic ex vivo liver perfusion:  results of a clinical trial.  Am J Transplant 2016; 17(4):1071-1080 28.

6. Livingstone SM, Andres A, Shapiro AM, Kneteman NN, Bigam DL.  Diaphragmatic hernia after living donor right hepatectomy:  proposal for a screening protocol.  Transplant Direct 2016;2(7):e84 

7. Ricordi C, Goldstein JS, Balamurugan AN, Szot GL, Kin T, Liu C, Czarniecki CW, Barbaro B, Bridges ND, Cano J, Clarke WR, Eggerman TL, Hunsicker LG, Kaufman DB, Khan A, Lafontant DE, Turgeon NA, Brandhorst D, Friberg AS, Lei J, Wang LK, Wilhelm JJ, Willits J, Zhang X, Hering BJ, Posselt AM, Shapiro AM.  NIH-sponsored clinical islet transplantation consortium phase 3 trial:  Manufacture of a complex cellular product at eight processing facilities.  Diabetes 2016 65(11): 2418-3428 

8. Pawlick RL, Wing J, Pepper AR, Bruni A, Abualhassen N, Rafiei Y, Gala-Lopez B, Bral M, Shapiro AM.  Reparixin, a CXCR1/2 inhibitor in islet allotransplantation.  Islets 2016;8(5): 115-124 

9. Bruni A, Pepper AR, Gala-Lopez B, Pawlick R, Abualhassan N, Crapo JD, Piganelli JD, Shapiro AM.  A novel redox-active metalloporphyrin reduces reactive oxygen species and inflammatory markers but does not improve marginal mass engraftment in a murine donation after circulatory death islet transplantation model. Islets 2016;8(4):e1190058 doi: 10/1080/19382014.2016.1190058 

10. Gala-Lopez BL, Kin T, O’Gorman D, Malcolm AJ, Pepper AR, Pawlick RL, Bruni A, Abualhassan N, Bral M, Jones C, Piganelli JD, Crapo JD, Shapiro AMJ. The metalloporphyrin BMX-010 in human islet isolation and clinical transplantation.  CellR4 2016;4(3):e2066 

11. Pepper AR, Pawlick R, Bruni A, Gala-Lopez B, Wink J, Rafiei Y, Bral M, Abualhassan N, Shapiro AM.  Harnessing the foreign body reaction in marginal mass device-less subcutaneous islet transplantation in mice.  Transplantation 2016; 100(7):1474-1479.

12. Wallner K, Shapiro AM, Senior PA, McCabe C.  Cost effectiveness and value of information analyses of islet cell transplantation in the management of “unstable” type 1 diabetes mellitus.  BMC Endocr Disord 2016;16:17 doi: 10.1186/s12902-016-0097-7.

13. 36. Hering BJ, Clarke WR, Bridges ND, Eggerman TL, Alejandro R, Bellin MD, Chaloner K, Czaniecki CW, Goldstein JS, Hunsicker LG, Kaufman DB, Korsgren O, Larsen CP, Luo X, Markmann JF, Naji A, Oberholzer J, Posselt AM, Rickels MR, Ricordi C, Robien MA, Senior PA, Shapiro AMJ, Stock PG, Turgeon NA for the Clinical Islet Transplantation Consortium.  Phase 3 trial of transplantation of human islets in type 1 diabetes complicated by severe hypoglycemia.  Diabetes Care 2016;39(7):1230-1240.(Jul)  

14. 37. Forbes S, Oram RA, Smith A, Lam A, Olateju T, Imes S, Malcolm AJ, Shapiro AM, Senior PA.  Validation of the BETA-2 score:  an improved tool to estimate beta-cell function after clinical islet transplantation utilizing a single fasting blood sample.  Am J Transplant 2016; doi: 10.1111/ajt.13807 (e-pub ahead of print).

15. 38. Shi W, Pawlick R, Bruni A, Rafiei Y, Pepper AR, Gala-Lopez B, Choi M, Malcolm A, Zemp RJ, Shapiro AMJ.  Photoacoustic imaging of angiogenesis in subdermal islet transplant sites.  Proc SPIE9708.  Photons Plus Ultrasound:  Imaging and Sensing 2016;97080Q;doi:10.1117/12.2211247

16. Lehmann-Werman R, Neima D, Zemmour H,Moss J, Magenheim J, Vaknin-Dembinsky A, Rubertson S, Nellgard B, Blennow K, Zetterberg H, Spaulding K, Haller MJ, Wasserfall CH, Schatz DA, Greenbaum CJ, Dorrell C, Grompe M, Zick A, Hubert A, Maoz M, Fendrich V, BArtsch DK, Golan T, Ben Sasson SA, Zamir G, Razin A, Cedar H, Shapiro AM, Glaser B, Shemer R, Dor Y.  Identification of tissue-specific cell death using methylation patterns of circulating DNA.  Proc Natl Acad Sci USA 2016;113(13)E1826-1834.mar

17. Helman A, Klochendler A, Azazmeh N, Gabal Y, Horwitz E, Anzi S, Swisa A, Candiotti R, Gramit RZ, Nevo Y, Fixler Y, Shreibman D, Zamir A, Tomovsky-Babeay S, Dai C, Glaser B, Powers AC, Shapiro AM, Magnuson MA, Dor Y, Ben-Porath I.  p16(Ink4a)-induced senescence of pancreatic beta cells enhances insulin secretion.  Nat Med 2016;22(4):412-420.feb

18. Matsushima H, Kuroki T, Adachi T, Kitasato A, Ono S, Tanaka T, Hirabaru M, Kuroshima N, Hirayama T, Sakai Y, Soyama A, Hidaka M, Takatsuki M, Kin T, Shapiro J, Eguchi S.  Human fibroblast sheet promotes human pancreatic islet survival and function in vitro.  Cell Transplant ;25 (8):15265-1537

19. Wang LJ, Kin T, O’Gorman D, Shapiro AM, Naziruddin B, Takita M, Levy MF, Posselt AM, Szot GL, Savari O, Barbaro B, McGarrigle J, Yeh CC, Oberholzer J, Lei J, Chen T, Lian M, Markmann JF, Alvarez A, Linetsky E, Ricordi C, Balamurugan AN, Loganathan G, Wilhelm JJ, Hering BJ, Bottino R, Trucco M, Liu C, Min Z, Li Y, Naji A, Fernandez LA, Ziemelis M, Danobeitia JS, Millis JM, Witkowski P.  A multicenter study:  North American islet donor score in donor pancreas selection for human islet isolation for transplantation.  Cell Transplant 2016;25(8):1515-1523.

20. Andres A, Livingstone S, Kin T, Campbell PM, Senior PA, Kneteman NM, Bigam D, Shapiro AM.  Islet after-failed-pancreas and pancreas-after-failed islet transplantation:  two complementary rescue strategies to control diabetes.  Islets 2016;e1126036.feb

21. Marmugi A, Parnis J, Chen X, Carmichael L, Hardy J, Mannan N, Marchetti P, ¬Piemonti L, Bosco D, Johnson P, Shapiro AM, Cruciani-Guglielmacci C, Magnan C, Ibberson M, Thorens B, Valdivia HH, Rutter GA, Leclerc I.  Sorcin links pancreatic β cell lipotoxicity to ER CA2+ stores.  Diabetes 2016;65(4):1009-1021feb.

22. Andres A, Kin T, O’Gorman D, Livingstone S, Bigam D, Kneteman N, Senior P, Shapiro AM.  Clinical islet isolation and transplantation outcomes with deceased cardiac death donors are similar to neurological determination of death donors.  Transplant Int 2016;29(1):34-40.

23. Robitaille K, Rourke JL, McBane JE, Fu A, Baird S, Du Q, Kin T, Shapiro AM, Screaton RA.  High-Throughput functional genomics identifies regulators of primary human beta cell proliferation.  J Biol Chem 2016;291(9):4614-4625.

24. Lyon J, Manning Fox JE, Spigelman AF, Kim R, Smith N, O’Gorman D, Kin T, Shapiro AM, Rajotte RV, MacDonald PE.  Research-focused isolation of human islets from donors with and without diabetes at the Alberta Diabetes Institute IsletCore.  Endocrinology 2016;157(2):560-569.

25. Gala-Lopez B, Pepper A, Pawlick R, O’Gorman D, Kin T, Bruni A, Abualhassan N, Bral M, Bautista A, Manning Fox J, MacDonald P, Young L, Shapiro J.  Anti-aging glycopeptide protects human islets against tacrolimus-related injury and facilitates engraftment in mice.  Diabetes 2016;65(2):451-462.

26. Brennan DC, Kopetskie HA, Sayre PH, Alejandro R Cagliero E, Shapiro AM, Goldstein JS, DesMarais MR, Booher S, Bianchine PJ.  Long-term follow-up of the Edmonton Protocol of Islet Transplantation in the United States.  Am J Transplant 2016;16(2):509-517.

2015

1. Moore SJ, Gala-Lopez BL, Pepper AR, Pawlick RL, Shapiro AJ. Bioengineered stem cells as an alternative for islet cell transplantation. World J Transplant. 2015 Mar 24;5(1):1-10. doi: 10.5500/wjt.v5.i1.1. Review. PubMed PMID: 25815266; PubMed Central PMCID: PMC4371156.

2. Pepper AR, Gala-Lopez B, Pawlick R, Merani S, Kin T, Shapiro AM. A prevascularized subcutaneous device-less site for islet and cellular transplantation. Nat Biotechnol. 2015 Apr 20. doi: 10.1038/nbt.3211. [Epub ahead of print] PubMed PMID: 25893782.

3. Senior PA, Bellin MD, Alejandro R, Yankey JW, Clarke WR, Qidwai JC, Schwieger TR, Eggerman TL, Robien MA, Rickels MR. Consistency of quantitative scores of hypoglycemia severity and glycemic lability and comparison with continuous glucose monitoring system measures in long-standing type 1 diabetes. Diabetes Technol Ther. 2015 Apr;17(4):235-42. doi: 10.1089/dia.2014.0289. Epub 2015 Jan 28. PubMed PMID: 25629445.

2014

1. Balamurugan AN, Naziruddin B, Lockridge A, Tiwari M, Loganathan G, Takita M, Matsumoto S, Papas K, Trieger M, Rainis H, Kin T, Kay TW, Wease S, Messinger S, Ricordi C, Alejandro R, Markmann J, Kerr-Conti J, Rickels MR, Liu C, Zhang X, Witkowski P, Posselt A, Maffi P, Secchi A, Berney T, O'Connell PJ, Hering BJ, Barton FB. Islet product characteristics and factors related to successful human islet transplantation from the Collaborative Islet Transplant Registry (CITR) 1999-2010. Am J Transplant. 2014 Nov;14(11):2595-606. doi: 10.1111/ajt.12872. Epub 2014 Oct 2. PubMed PMID: 25278159; PubMed Central PMCID: PMC4282081.

2. Pawlick R, Gala-Lopez B, Pepper AR, McCall M, Ziff O, Shapiro AM. The combination of anti-NKG2D and CTLA-4 Ig therapy prolongs islet allograft survival in a murine model. Am J Transplant. 2014 Oct;14(10):2367-74. doi: 10.1111/ajt.12838. Epub 2014 Sep 1. Erratum in: Am J Transplant. 2014 Nov;14(11):2674. PubMed PMID: 25179027.

3. Kitzmann JP, Pepper AR, Gala-Lopez B, Pawlick R, Kin T, O'Gorman D, Mueller KR, Gruessner AC, Avgoustiniatos ES, Karatzas T, Szot GL, Posselt AM, Stock PG, Wilson JR, Shapiro AM, Papas KK. Human islet viability and function is maintained during high-density shipment in silicone rubber membrane vessels. Transplant Proc. 2014 Jul-Aug;46(6):1989-91. doi: 10.1016/j.transproceed.2014.06.002. PubMed PMID: 25131090; PubMed Central PMCID: PMC4169700.

4. Kitzmann JP, O'Gorman D, Kin T, Gruessner AC, Senior P, Imes S, Gruessner RW, Shapiro AM, Papas KK. Islet oxygen consumption rate dose predicts insulin independence for first clinical islet allotransplants. Transplant Proc. 2014 Jul-Aug;46(6):1985-8. doi: 10.1016/j.transproceed.2014.06.001. PubMed PMID: 25131089; PubMed Central PMCID: PMC4170186.

5. Bruni A, Gala-Lopez B, Pepper AR, Abualhassan NS, Shapiro AJ. Islet cell transplantation for the treatment of type 1 diabetes: recent advances and future challenges. Diabetes Metab Syndr Obes. 2014 Jun 23;7:211-23. doi: 10.2147/DMSO.S50789. eCollection 2014. Review. PubMed PMID: 25018643; PubMed Central PMCID: PMC4075233.

6. Andres A, Kin T, O'Gorman D, Bigam D, Kneteman N, Senior P, Shapiro AJ. Impact of adverse pancreatic injury at surgical procurement upon islet isolation outcome. Transpl Int. 2014 Nov;27(11):1135-42. doi: 10.1111/tri.12392. Epub 2014 Aug 24. PubMed PMID: 24977306.

7. McCall M, Shapiro AM. Islet cell transplantation. Semin Pediatr Surg. 2014 Apr;23(2):83-90. doi: 10.1053/j.sempedsurg.2014.03.006. Epub 2014 May 2. PubMed PMID: 24931353.

8. Al-Adra DP, Gill RS, Imes S, O'Gorman D, Kin T, Axford SJ, Shi X, Senior PA, Shapiro AM. Single-donor islet transplantation and long-term insulin independence in select patients with type 1 diabetes mellitus. Transplantation. 2014 Nov 15;98(9):1007-12. doi: 10.1097/TP.0000000000000217. PubMed PMID: 4911037.

2013

1. Canadian Diabetes Association Clinical Practice Guidelines Expert Committee, Paty BW, Koh A, Senior P. Pancreas and islet transplantation. Can J Diabetes. 2013 Apr;37 Suppl 1:S94-6. doi: 10.1016/j.jcjd.2013.01.028. Epub 2013 Mar 26. PubMed PMID: 24070973.

2. Miranda PM, Mohan V, Ganthimathy S, Anjana RM, Gunasekaran S, Thiagarajan V, Churchill TA, Kin T, Shapiro AM, Lakey JR. Human islet mass, morphology, and survival after cryopreservation using the Edmonton protocol. Islets. 2013 Sep-Dec;5(5):188-95. doi: 10.4161/isl.26304. PubMed PMID: 24759005; PubMed Central PMCID: PMC4010570.

3. Pepper AR, Gala-Lopez B, Ziff O, Shapiro AJ. Current status of clinical islet transplantation. World J Transplant. 2013 Dec 24;3(4):48-53. doi: 10.5500/wjt.v3.i4.48. PubMed PMID: 24392308; PubMed Central PMCID: PMC3879523.

4. Pepper AR, Gala-Lopez B, Ziff O, Shapiro AM. Revascularization of transplanted pancreatic islets and role of the transplantation site. Clin Dev Immunol. 2013;2013:352315. doi: 10.1155/2013/352315. Epub 2013 Sep 9. Review. PubMed PMID: 24106517; PubMed Central PMCID: PMC3782812.

5. Gala-Lopez B, Pepper AR, Shapiro AM. Biologic agents in islet transplantation. Curr Diab Rep. 2013 Oct;13(5):713-22. doi: 10.1007/s11892-013-0414-8. Review. PubMed PMID: 23918618.

6. O'Gorman D, Kin T, Pawlick R, Imes S, Senior PA, Shapiro AM. Clinical islet isolation outcomes with a highly purified neutral protease for pancreas dissociation. Islets. 2013 May-Jun;5(3):111-5. doi: 10.4161/isl.25222. Epub 2013 Jun 11. PubMed PMID: 23756701; PubMed Central PMCID: PMC4049837.

7. Koh A, Imes S, Shapiro AM, Senior PA. Successful treatment of brittle diabetes following total pancreatectomy by islet allotransplantation: a case report. JOP. 2013 Jul 10;14(4):428-31. doi: 10.6092/1590-8577/1440. PubMed PMID: 23846941.

8.  Shapiro AM. Immune antibody monitoring predicts outcome in islet transplantation. Diabetes. 2013 May;62(5):1377-8. doi: 10.2337/db13-0019. PubMed PMID: 23613556; PubMed Central PMCID: PMC3636639.

9. Jackson S, Mager DR, Bhargava R, Ackerman T, Imes S, Hubert G, Koh A, Shapiro AM, Senior PA. Long-term follow-up of hepatic ultrasound findings in subjects with magnetic resonance imaging defined hepatic steatosis following clinical islet transplantation: a case-control study. Islets. 2013 Jan-Feb;5(1):16-21. doi: 10.4161/isl.24058. Epub 2013 Jan 1. PubMed PMID: 23514958; PubMed Central PMCID: PMC3662378.

10. Gala-Lopez B, Kin T, O'Gorman D, Pepper AR, Senior P, Humar A, Shapiro AM. Microbial contamination of clinical islet transplant preparations is associated with very low risk of infection. Diabetes Technol Ther. 2013 Apr;15(4):323-7. doi: 10.1089/dia.2012.0297. Epub 2013 Feb 25. PubMed PMID: 23438305; PubMed Central PMCID: PMC3661032.

11. do Amaral AS, Pawlick RL, Rodrigues E, Costal F, Pepper A, Galvão FH, Correa-Giannella ML, Shapiro AM. Glutathione ethyl ester supplementation during pancreatic islet isolation improves viability and transplant outcomes in a murine marginal islet mass model. PLoS One. 2013;8(2):e55288. doi:10.1371/journal.pone.0055288. Epub 2013 Feb 12. PubMed PMID: 23424628; PubMed Central PMCID: PMC3570543.

2012

1. Silva M, Humar A, Shapiro AM, Senior P, Hoschler K, Baluch A, Wilson LE, Kumar D. Humoral immune response following seasonal influenza vaccine in islet transplant recipients. Cell Transplant. 2013;22(3):469-76. doi: 10.3727/096368912X656135. Epub 2012 Sep 21. PubMed PMID: 23006439.

2. Al-Adra DP, Pawlick R, Shapiro AM, Anderson CC. Targeting cells causing split tolerance allows fully allogeneic islet survival with minimal conditioning in NOD mixed chimeras. Am J Transplant. 2012 Dec;12(12):3235-45. doi: 10.1111/j.1600-6143.2012.04260.x. Epub 2012 Sep 13. PubMed PMID: 22974315.

3. McCall M, Shapiro AM. Update on islet transplantation. Cold Spring Harb Perspect Med. 2012 Jul;2(7):a007823. doi: 10.1101/cshperspect.a007823. Review. PubMed PMID: 22762022; PubMed Central PMCID: PMC3385934. 

4. Barton FB, Rickels MR, Alejandro R, Hering BJ, Wease S, Naziruddin B, Oberholzer J, Odorico JS, Garfinkel MR, Levy M, Pattou F, Berney T, Secchi A, Messinger S, Senior PA, Maffi P, Posselt A, Stock PG, Kaufman DB, Luo X, Kandeel F, Cagliero E, Turgeon NA, Witkowski P, Naji A, O'Connell PJ, Greenbaum C, Kudva YC, Brayman KL, Aull MJ, Larsen C, Kay TW, Fernandez LA, Vantyghem MC, Bellin M, Shapiro AM. Improvement in outcomes of clinical islet transplantation: 1999-2010. Diabetes Care. 2012 Jul;35(7):1436-45. doi: 10.2337/dc12-0063. PubMed PMID: 22723582; PubMed Central PMCID: PMC3379615.

5. McCall MD, Maciver AM, Kin T, Emamaullee J, Pawlick R, Edgar R, Shapiro AM. Caspase inhibitor IDN6556 facilitates marginal mass islet engraftment in a porcine islet autotransplant model. Transplantation. 2012 Jul 15;94(1):30-5. doi: 10.1097/TP.0b013e318257745d. PubMed PMID: 22706322.

6. Yeung TY, Seeberger KL, Kin T, Adesida A, Jomha N, Shapiro AM, Korbutt GS. Human mesenchymal stem cells protect human islets from pro-inflammatory cytokines. PLoS One. 2012;7(5):e38189. doi: 10.1371/journal.pone.0038189. Epub 2012 May 30. PubMed PMID: 22666480; PubMed Central PMCID: PMC3364233.

7. Merani S, Famulski KS, Ramassar V, Shapiro AJ, Halloran PF. Characterization of the transcriptome in isolated and transplanted mouse pancreatic islets: associations with engraftment and dysfunction. Islets. 2012 Mar-Apr;4(2):158-66. doi: 10.4161/isl.19770. Epub 2012 Mar 1. PubMed PMID: 22653155.

8. Shapiro AM. Islet transplantation in type 1 diabetes: ongoing challenges, refined procedures, and long-term outcome. Rev Diabet Stud. 2012 Winter;9(4):385-406. doi: 10.1900/RDS.2012.9.385. Epub 2012 Dec 28. Review. PubMed PMID: 23804275; PubMed Central PMCID: PMC3740705.

9. Kin T, O'Gorman D, Schroeder A, Onderka C, Richer B, Rosichuk S, Zhai X, Shapiro AM. Human islet distribution program for basic research at a single center. Transplant Proc. 2011 Nov;43(9):3195-7. doi: 10.1016/j.transproceed.2011.10.003. PubMed PMID: 22099755.

10. Shapiro AM. Strategies toward single-donor islets of Langerhans transplantation. Curr Opin Organ Transplant. 2011 Dec;16(6):627-31. doi: 10.1097/MOT.0b013e32834cfb84. Review. PubMed PMID: 22068022; PubMed Central PMCID: PMC3268080.

11. McCall M, Pawlick R, Kin T, Shapiro AM. Anakinra potentiates the protective effects of etanercept in transplantation of marginal mass human islets in immunodeficient mice. Am J Transplant. 2012 Feb;12(2):322-9. doi: 10.1111/j.1600-6143.2011.03796.x. Epub 2011 Nov 4. PubMed PMID: 22053751.

2011

1. Senior PA, Koh A, Shapiro AM. Remission of alopecia universalis following successful clinical islet transplantation. Am J Transplant. 2011 Nov;11(11):2536-7. doi: 10.1111/j.1600-6143.2011.03758.x. Epub 2011 Sep 22. PubMed PMID: 21951462.

2. Merani S, McCall M, Pawlick RL, Edgar RL, Davis J, Toso C, Emamaullee JA, Kin T, Shapiro AM. AEB071 (sotrastaurin) does not exhibit toxic effects on human islets in vitro, nor after transplantation into immunodeficient mice. Islets. 2011 Nov-Dec;3(6):338-43. doi: 10.4161/isl.3.6.17766. Epub 2011 Nov 1. PubMed PMID: 21934354.

3. Gala-Lopez BL, Senior PA, Koh A, Kashkoush SM, Kawahara T, Kin T, Humar A, Shapiro AM. Late cytomegalovirus transmission and impact of T-depletion in clinical islet transplantation. Am J Transplant. 2011 Dec;11(12):2708-14. doi: 10.1111/j.1600-6143.2011.03724.x. Epub 2011 Sep 11. PubMed PMID: 21906252.

4. McCall MD, Pawlick R, Shapiro AM. Resveratrol fails to improve marginal mass engraftment of transplanted islets of Langerhans in mice. Islets. 2011 Sep-Oct;3(5):241-5. doi: 10.4161/isl.3.5.16698. PubMed PMID: 21904116.

5. Kawahara T, Kin T, Kashkoush S, Gala-Lopez B, Bigam DL, Kneteman NM, Koh A, Senior PA, Shapiro AM. Portal vein thrombosis is a potentially preventable complication in clinical islet transplantation. Am J Transplant. 2011 Dec;11(12):2700-7. doi: 10.1111/j.1600-6143.2011.03717.x. Epub 2011 Aug 29. PubMed PMID: 21883914; PubMed Central PMCID: PMC3226916.

6. Kawahara T, Kin T, Shapiro AM. A comparison of islet autotransplantation with allotransplantation and factors elevating acute portal pressure in clinical islet transplantation. J Hepatobiliary Pancreat Sci. 2012 May;19(3):281-8. doi: 10.1007/s00534-011-0441-2. PubMed PMID: 21879320.

7. Shapiro AM. State of the art of clinical islet transplantation and novel protocols of immunosuppression. Curr Diab Rep. 2011 Oct;11(5):345-54. doi: 10.1007/s11892-011-0217-8. Review. PubMed PMID: 21830042.

8. McCall MD, Pawlick R, Shapiro AM. Resveratrol fails to improve marginal mass engraftment of transplanted islets of Langerhans in mice. Islets. 2011 Sep 1;3(5). [Epub ahead of print] PubMed PMID: 21757994.

9. ElSaadany S, Tyshenko MG, Oraby T, Malcolm AJ, Senior P, Shapiro AM, Catford A, Salem T, Wu J, Krewski D, Rohwer RG. The risk to human islet cell transplant recipients of acquiring variant Creutzfeldt-Jakob disease: a provisional quantitative risk assessment. Transplantation. 2011 Jul 15;92(1):e2-4. doi: 10.1097/TP.0b013e31821eabab. PubMed PMID: 21691192.

10. McCall M, Toso C, Emamaullee J, Pawlick R, Edgar R, Davis J, Maciver A, Kin T, Arch R, Shapiro AM. The caspase inhibitor IDN-6556 (PF3491390) improves marginal mass engraftment after islet transplantation in mice. Surgery. 2011 Jul;150(1):48-55. doi: 10.1016/j.surg.2011.02.023. Epub 2011 May 18. PubMed PMID: 21596412.

11. Zuk DM, Koh A, Imes S, Shapiro AM, Senior PA. Three cases of alopecia following clinical islet transplantation. Am J Transplant. 2011 Jan;11(1):163-8. doi: 10.1111/j.1600-6143.2010.03348.x. PubMed PMID: 21199356; PubMed Central PMCID: PMC3052626.

2010

1. Kin T, O'Gorman D, Senior P, Shapiro AM. Experience of islet isolation without neutral protease supplementation. Islets. 2010 Sep-Oct;2(5):278-82. Epub 2010 Sep 1. PubMed PMID: 21099325; PubMed Central PMCID: PMC3230559.

2. Kin T, Shapiro AM. Surgical aspects of human islet isolation. Islets. 2010 Sep-Oct;2(5):265-73. Epub 2010 Sep 1. Review. PubMed PMID: 21099323; PubMed Central PMCID: PMC3230558.

3. Kin T, Shapiro J. Partial dorsal agenesis accompanied with circumportal pancreas in a donor for islet transplantation. Islets. 2010 May-Jun;2(3):146-8. doi: 10.4161/isl.2.3.11715. PubMed PMID: 21099308.

4. Lacotte S, Berney T, Shapiro AJ, Toso C. Immune monitoring of pancreatic islet graft: towards a better understanding, detection and treatment of harmful events. Expert Opin Biol Ther. 2011 Jan;11(1):55-66. doi: 10.1517/14712598.2011.536530. Epub 2010 Nov 12. Review. PubMed PMID: 21073277.

5. Toso C, Pawlick R, Lacotte S, Edgar R, Davis J, McCall M, Morel P, Mentha G, Berney T, Shapiro AM. Detecting rejection after mouse Islet transplantation utilizing islet protein-stimulated ELISPOT. Cell Transplant. 2011;20(6):955-62. doi: 10.3727/096368910X539137. Epub 2010 Nov 5. PubMed PMID: 21054945.

6. O'Gorman D, Kin T, Imes S, Pawlick R, Senior P, Shapiro AM. Comparison of human islet isolation outcomes using a new mammalian tissue-free enzyme versus collagenase NB-1. Transplantation. 2010 Aug 15;90(3):255-9. doi: 10.1097/TP.0b013e3181e117ce. PubMed PMID: 20463640.

7. Emamaullee JA, Davis J, Pawlick R, Toso C, Merani S, Cai SX, Tseng B, Shapiro AM. Caspase inhibitor therapy synergizes with costimulation blockade to promote indefinite islet allograft survival. Diabetes. 2010 Jun;59(6):1469-77. doi: 10.2337/db09-0502. Epub 2010 Mar 23. PubMed PMID: 20332344; PubMed Central PMCID: PMC2874708.

8. Low G, Hussein N, Owen RJ, Toso C, Patel VH, Bhargava R, Shapiro AM. Role of imaging in clinical islet transplantation. Radiographics. 2010 Mar;30(2):353-66. doi: 10.1148/rg.302095741. Review. Erratum in: Radiographics. 2010 May-Jun;30(3):844. PubMed PMID: 20228322.

9. Kin T. Islet isolation for clinical transplantation. Adv Exp Med Biol. 2010;654:683-710. doi: 10.1007/978-90-481-3271-3_30. Review. PubMed PMID: 20217520.

10. Toso C, Merani S, Bigam DL, Shapiro AM, Kneteman NM. Sirolimus-based immunosuppression is associated with increased survival after liver transplantation for hepatocellular carcinoma. Hepatology. 2010 Apr;51(4):1237-43. doi: 10.1002/hep.23437. PubMed PMID: 20187107.

11. Koh A, Senior P, Salam A, Kin T, Imes S, Dinyari P, Malcolm A, Toso C, Nilsson B, Korsgren O, Shapiro AM. Insulin-heparin infusions peritransplant substantially improve single-donor clinical islet transplant success. Transplantation. 2010 Feb 27;89(4):465-71. doi: 10.1097/TP.0b013e3181c478fd. PubMed PMID: 20177350.

12. Koh A, Imes S, Kin T, Dinyari P, Malcolm A, Toso C, Shapiro AM, Senior P. Supplemental islet infusions restore insulin independence after graft dysfunction in islet transplant recipients. Transplantation. 2010 Feb 15;89(3):361-5. doi: 10.1097/TP.0b013e3181bcdbe8. PubMed PMID: 20145529.

2009

1. Toso C, Isse K, Demetris AJ, Dinyari P, Koh A, Imes S, Kin T, Emamaullee J, Senior P, Shapiro AM. Histologic graft assessment after clinical islet transplantation. Transplantation. 2009 Dec 15;88(11):1286-93. doi: 10.1097/TP.0b013e3181bc06b0. PubMed PMID: 19996928.

2. Toso C, McCall M, Emamaullee J, Merani S, Davis J, Edgar R, Pawlick R, Kin T, Knudsen LB, Shapiro AM. Liraglutide, a long-acting human glucagon-like peptide 1 analogue, improves human islet survival in culture. Transpl Int. 2010 Mar 1;23(3):259-65. doi: 10.1111/j.1432-2277.2009.00984.x. Epub 2009 Oct 12. PubMed PMID: 19821955.

3. McCall MD, Toso C, Baetge EE, Shapiro AM. Are stem cells a cure for diabetes?Clin Sci (Lond). 2009 Oct 12;118(2):87-97. doi: 10.1042/CS20090072. Review. PubMed PMID: 19807695.

4. CITR Research Group. 2007 update on allogeneic islet transplantation from the Collaborative Islet Transplant Registry (CITR). Cell Transplant. 2009;18(7):753-67. doi: 10.3727/096368909X470874. Epub 2009 Sep 28. PubMed PMID: 19796497.

5. Kin T, Shapiro AM. Circumportal pancreas and islet isolation. Surgery. 2009 Jul;146(1):126-7. doi: 10.1016/j.surg.2008.04.017. Epub 2008 Aug 10. PubMed PMID: 19541017.

6. Kin T, O'Gorman D, Zhai X, Pawlick R, Imes S, Senior P, Shapiro AM. Nonsimultaneous administration of pancreas dissociation enzymes during islet isolation. Transplantation. 2009 Jun 15;87(11):1700-5. doi: 10.1097/TP.0b013e3181a60240. PubMed PMID: 19502963.

7. Emamaullee J, Toso C, Merani S, Shapiro AM. Costimulatory blockade with belatacept in clinical and experimental transplantation - a review. Expert Opin Biol Ther. 2009 Jun;9(6):789-96. doi: 10.1517/14712590902942284 . Review. PubMed PMID: 19426116.

8. Truong W, Hancock WW, Plester JC, Merani S, Rayner DC, Thangavelu G, Murphy KM, Anderson CC, Shapiro AM. BTLA targeting modulates lymphocyte phenotype, function, and numbers and attenuates disease in nonobese diabetic mice. J Leukoc Biol. 2009 Jul;86(1):41-51. doi: 10.1189/jlb.1107753. Epub 2009 Apr 21. PubMed PMID: 19383625.

9. Owen RJ, Mercer JR, Al-Saif F, Molinari M, Ashforth RA, Rajotte RV, Conner-Spady B, Shapiro AM. Portal vein embolization with radiolabeled polyvinyl alcohol particles in a swine model: hepatic distribution and implications for pancreatic islet cell transplantation. Cardiovasc Intervent Radiol. 2009 May;32(3):499-507. doi: 10.1007/s00270-009-9544-4. Epub 2009 Mar 28. PubMed PMID: 19330373.

10. Merani S, Edgar RL, Toso C, Emamaullee J, Thiesen A, Shapiro AM. AEB-071 has minimal impact on onset of autoimmune diabetes in NOD mice. Autoimmunity. 2009 Mar;42(3):242-8. doi: 10.1080/08916930802587950. PubMed PMID: 19301207.

11. Emamaullee JA, Davis J, Merani S, Toso C, Elliott JF, Thiesen A, Shapiro AM. Inhibition of Th17 cells regulates autoimmune diabetes in NOD mice. Diabetes. 2009 Jun;58(6):1302-11. doi: 10.2337/db08-1113. Epub 2009 Mar 16. PubMed PMID: 19289457; PubMed Central PMCID: PMC2682686.

12. Alfadhli E, Koh A, Albaker W, Bhargava R, Ackerman T, McDonald C, Ryan EA, Shapiro AM, Senior PA. High prevalence of ovarian cysts in premenopausal women receiving sirolimus and tacrolimus after clinical islet transplantation. Transpl Int. 2009 Jun;22(6):622-5. doi: 10.1111/j.1432-2277.2009.00839.x. Epub 2009 Feb 2. PubMed PMID: 19207188.

13. Merani S, Pawlick RL, Edgar RL, Toso C, Emamaullee J, Anderson CC, Shapiro AM. Protein kinase C inhibitor, AEB-071, acts complementarily with cyclosporine to prevent islet rejection in rats. Transplantation. 2009 Jan 15;87(1):59-65. doi: 10.1097/TP.0b013e31819198b4. PubMed PMID: 19136892.

2008

1. Kin T, O'Neil JJ, Pawlick R, Korbutt GS, Shapiro AM, Lakey JR. The use of an approved biodegradable polymer scaffold as a solid support system for improvement of islet engraftment. Artif Organs. 2008 Dec;32(12):990-3. doi:10.1111/j.1525-1594.2008.00688.x. PubMed PMID: 19133030.

2. Emamaullee JA, Merani S, Toso C, Kin T, Al-Saif F, Truong W, Pawlick R, DavisJ, Edgar R, Lock J, Bonner-Weir S, Knudsen LB, Shapiro AM. Porcine marginal mass islet autografts resist metabolic failure over time and are enhanced by earlytreatment with liraglutide. Endocrinology. 2009 May;150(5):2145-52. doi:10.1210/en.2008-1116. Epub 2009 Jan 8. PubMed PMID: 19131571.

3. Alejandro R, Barton FB, Hering BJ, Wease S; Collaborative Islet Transplant Registry Investigators. 2008 Update from the Collaborative Islet Transplant Registry. Transplantation. 2008 Dec 27;86(12):1783-8. doi:10.1097/TP.0b013e3181913f6a. PubMed PMID: 19104422.

4. Koh A, Kin T, Imes S, Shapiro AM, Senior P. Islets isolated from donors with elevated HbA1c can be successfully transplanted. Transplantation. 2008 Dec 15;86(11):1622-4. doi: 10.1097/TP.0b013e31818c2559. PubMed PMID: 19077899.

5. Merani S, Toso C, Emamaullee J, Shapiro AM. Optimal implantation site for pancreatic islet transplantation. Br J Surg. 2008 Dec;95(12):1449-61. doi: 10.1002/bjs.6391. Review. PubMed PMID: 18991254.

6. Fiorina P, Shapiro AM, Ricordi C, Secchi A. The clinical impact of islet transplantation. Am J Transplant. 2008 Oct;8(10):1990-7. doi: 10.1111/j.1600-6143.2008.02353.x. Review. PubMed PMID: 18828765.

7. Shapiro AM. Islet transplantation--the imperative need for continued clinical trials. Nat Clin Pract Nephrol. 2008 Dec;4(12):662-3. doi: 10.1038/ncpneph0964. Epub 2008 Sep 30. PubMed PMID: 18825154.

8. Toso C, Edgar R, Pawlick R, Emamaullee J, Merani S, Dinyari P, Mueller TF, Shapiro AM, Anderson CC. Effect of different induction strategies on effector, regulatory and memory lymphocyte sub-populations in clinical islet transplantation. Transpl Int. 2009 Feb;22(2):182-91. doi: 10.1111/j.1432-2277.2008.00746.x. Epub 2008 Aug 15. PubMed PMID: 18713144.

9. Kin T, Zhai X, O'Gorman D, Shapiro AM. Detrimental effect of excessive collagenase class II on human islet isolation outcome. Transpl Int. 2008 Nov;21(11):1059-65. doi: 10.1111/j.1432-2277.2008.00734.x. Epub 2008 Aug 1. PubMed PMID: 18680482.

10. Ricordi C, Hering BJ, Shapiro AM; Clinical Islet Transplantation Consortium. Beta-cell transplantation for diabetes therapy. Lancet. 2008 Jul 5;372(9632):27-8; author reply 29-30. doi: 10.1016/S0140-6736(08)60984-8. PubMed PMID: 18603151.

11. Kin T, Senior P, O'Gorman D, Richer B, Salam A, Shapiro AM. Risk factors for islet loss during culture prior to transplantation. Transpl Int. 2008 Nov;21(11):1029-35. doi: 10.1111/j.1432-2277.2008.00719.x. Epub 2008 Jun 18. PubMed PMID: 18564983.

12. Merani S, Truong W, Emamaullee JA, Toso C, Knudsen LB, Shapiro AM. Liraglutide, a long-acting human glucagon-like peptide 1 analog, improves glucose homeostasis in marginal mass islet transplantation in mice. Endocrinology. 2008 Sep;149(9):4322-8. doi: 10.1210/en.2008-0501. Epub 2008 May 29. PubMed PMID: 18511515.

13. Emamaullee JA, Merani S, Larsen CP, Shapiro AM. Belatacept and basiliximab diminish human antiporcine xenoreactivity and synergize to inhibit alloimmunity. Transplantation. 2008 Jan 15;85(1):118-24. doi: 10.1097/01.tp.0000296832.92128.94. PubMed PMID: 18192921.

14. Truong W, Plester JC, Hancock WW, Kaye J, Merani S, Murphy KM, Murphy TL, Anderson CC, Shapiro AM. Negative and positive co-signaling with anti-BTLA (PJ196) and CTLA4Ig prolongs islet allograft survival. Transplantation. 2007 Nov 27;84(10):1368-72. Erratum in: Transplantation. 2008 Jun 27;85(12):1872-3. PubMed PMID: 18049125.

15. Truong W, Plester JC, Hancock WW, Merani S, Murphy TL, Murphy KM, Kaye J, Anderson CC, Shapiro AM. Combined coinhibitory and costimulatory modulation with anti-BTLA and CTLA4Ig facilitates tolerance in murine islet allografts. Am J Transplant. 2007 Dec;7(12):2663-74. Erratum in: Am J Transplant. 2008 Feb;8(2):471. PubMed PMID: 17983390.

16. Emamaullee JA, Davis J, Pawlick R, Merani S, Cai S, Tseng B, Shapiro AMJ. The caspase selective inhibitor EP1013 augments human islet graft function and longevity in marginal mass islet transplantation in mice. Published ahead of print, Diabetes, March 20, 2008.

2007

• 1. Shapiro AMJ, Bowker S, Dinyari P, Paty B, Ryan EA, Senior P, Johnson JA. Quality of Life After Islet Transplant: Impact of the Number of Islet Infusions and Metabolic Outcome. Transplantation 2007;84(5):664-666.

  2. Campbell PM, Senior PA, Salam A, LaBranche K, Bigam DL, Kneteman NM, Imes S, Halpin A, Ryan EA, Shapiro AMJ. High Risk of Sensitization After Failed Islet Transplantation. Am J Transplant 2007;7(10):2311-2317

  3. Lakey JRT, Kin T, Warnock GL, Shapiro AMJ, Tsapongas P, Imes S, Korbutt GS, Kneteman NM, Rajotte RV, Ryan EA. Long-Term Graft Function After Allogeneic Islet Transplantation. Cell Transplant 2007;16(4):441-446.

  4. Campbell PM, Salam A, Ryan EA, Senior P, Paty BW, Bigam D, McCready T, Halpin A, Imes S, Al Saif F, Lakey JRT, Shapiro AMJ. Pretransplant HLA Antibodies Are Associated with Reduced Graft Survival After Clinical Islet Transplantation. Am J Transplant 2007;7:1242-1248.

     1. Kin T, Rosichuk S, Shapiro AM, Lakey JR. Detection of microbial contamination during human islet isolation. Cell Transplant 2007;16(1):9-13.

  5. MWalter M, McDonald CG, Paty BW, Shapiro AMJ, Ryan EA, Senior PA. Prevalence of autoimmune diseases in islet transplant candidates with severe hypoglycaemia and glycaemic lability: previously undiagnosed celiac and autoimmune thyroid disease is identified by screening. Diabetic Medicine 2007;24:161-165.

  6. Suchak AA, O’Kelly K, Al Saif F, Shapiro AMJ, Owen RJ. Hepatic Artery-Portal Venous Fistula After Percutaneous Intraportal Islet Cell Transplant. (Letter to the Editor) Transplantation 2007;83(5):669-670.

  7. Kin T, Zhai X, Murdoch TB, Salam A, Shapiro AMJ, Lakey JRT. Enhancing the Success of Human Islet Isolation Through Optimization and Characterization of Pancreas Dissociation Enzyme. Am J Transplant 2007;7:1-8.

  8. Senior PA, Zeman M, Paty BW, Ryan EA, Shapiro AMJ. Changes in Renal Function after Clinical Islet Transplantation: Four-Year Observational Study. Am J Transplant 2007;7:91-98.

  9. SKin T, Johnson PRV, Shapiro AMJ, Lakey JRT. Factors Influencing the Collagenase Digestion Phase of Human Islet Isolation. Transplantation 2007;83:7-12.

2006

• 1. Matsumoto S, Okitsu T, Iwanaga Y, Noguchi H, Nagata H, Yonekawa Y, Liu X, Kamiya H, Ueda M, Hatanaka N, Kobayashi N, Yamada Y, Miyakawa S, Seino Y, Shapiro AMJ and Tanaka K. Follow-up Study of the First Successful Living Donor Islet Transplantation. Transplantation 2006;82(12): 1629–1633

  2. Kin T, Mirbolooki M, Salehi P, Tsukada M, O’Gorman D, Imes S, Ryan EA, Shapiro AMJ, Lakey JRT. Islet Isolation and Transplantation Outcomes of Pancreas Preserved with University of Wisconsin Solution Versus Two-Layer Method Using Peroxygenated Perfluorocarbon. Transplantation 2006;82(10:1286-1290

  3. Shapiro AMJ, Ricordi C, Hering BJ, Auchincloss H, Lindblad R, Robertson RP, Secchi A, Brendel MD, Berney T, Brennan DC, Cagliero E, Alejandro R, Ryan EA, Diercurio B, Morel P, Polonsky KS, Reems J, Bretzel RG, Bertuzzi F, Froud T, Kandaswamy R, Sutherland DER, EisenbarthG, Segal M, Preiksaitis J, Korbutt GS, Barton FB, Viviano L, Seyfert-Margolis V, Bluestone J, Lakey JRT. International Multicenter Trial of Islet Transplantation with the Edmonton Protocol in Type 1 Diabetes. New Engl J Med 2006;355(13)1318-30. Editorial by Dr. J.S. Bromberg.

  4. Berney T, Mamin A, Shapiro AMJ, Ritz-Laser B, Brulhart M-C, Toso C, Demyulder-Mischler S, Armanet M, Baertschiger R, Wojtusciszyn A, Benhamou P-Y, Bosco D, Morel P, Philippe J. Detection of Insulin mRNA in the Peripheral Blood After Human Islet Transplantation Predicts Deterioration of Metabolic Control. Am J Transplant 2006;6:1704-1711.

  5. Paty BW, Senior PA, Lakey JFRT, Shapiro AMJ, Ryan EA. Assessment of Glycemic Control After Islet Transplantation Using the Continuous Glucose Monitor in insulin-Independent Versus Insulin-Requiring Type 1 Diabetes Subjects. Diabetes Technol Ther 2006;8(2):165-172.

     1. Alsaif F, Molinari M, Al-Masloom A, Lakey JRT, Kin T, Shapiro AMJ. Pancreatic Islet Autotransplantation with Completion Pancreatectomy in the Management of Uncontrolled Pancreatic Fistula After Whipple Resection for Ampullary Adenocarcinoma (Letter to the Editor). Pancreas 2006;32(4):430-431.

  6. Mirbolooki M, Shapiro AMJ, Lakey JRT. A Perspective on Clinical Islet Transplantation: Past, Present and Developments for Future. Immun. Endoc & Metab. Agents in Med. Chem 2006;6:191-208.

  7. Salehi P, Mirbolooki M, Kin T, Tsujimura T, Shapiro AM, Churchill TA, Lakey JR. Ameliorating injury during preservation and isolation of human islets using the two-layer method with perfluorocarbon and UW solution. Cell Transplant 2006;15(2):187-94.

  8. Merani S, Shapiro AMJ. Current Status of pancreatic islet transplantation. Clin Sci 2006;110:611-625.

  9. Kin T, Murdoch TB, Shapiro AM, Lakey JR. Estimation of pancreas weight from donor variables. Cell Transplant. 2006;15(2):181-185.

  10. Shapiro AMJ. Transplanting Islets for Diabetes (Feature Article). Scientist. 2006;20(5):43.

  11. Truong W, Shapiro AMJ. Progress in Islet Transplantation in Patients with Type 1 Diabetes Mellitus. Treat Endocrinol. 2006;5(3):147-158.

  12. Ryan EA, Bigam D, Shapiro AM. Current indications for pancreas or islet transplant. Diabetes Obes Metab. 2006;8(1):1-7.

  13. Seeberger KL, Dufour JM, Shapiro AMJ, Lakey JR, Rajotte RV, Korbutt GS. Expansion of mesenchymal stem cells from human pancreatic ductal epithelium. Lab Invest. 2006;86;141-153.

  14. Nanji SA, Shapiro AM. Advances in pancreatic islet transplantation in humans. Diabetes Obes Metab. 2006; 8(1):15-25.

2005

• 1. Villiger P, Ryan E, Owen R, O’Kelly K, Oberholzer J, Al Saif F, Kin T, Wang H, Larsen I, Blitz SL, Menon V, Senior P, Bigam DL, Paty BW, Kneteman NM, Lakey JRT, Shapiro AMJ. Prevention of Bleeding After Islet Transplantation – Lessons Learned from a Multivariate Analysis of 132 Cases at a Single Institution. Am J Transpl 2005;5: 2992-2998.

  2. O’Gorman D, Kin T, Murdoch T, Richer B, McGhee-Wilson D, Ryan EA, Shapiro AMJ, Lakey JR. The Standardization of Pancreatic Donors for Islet Isolations. Transplantation2005 Sep 27;80(6):801-6.

  3. Barnett MJ, Zhai XW, Legatt DF, Cheng SB, Shapiro AMJ, Lakey JR. Quantitative Assessment of Collagenase Blends for Human Islet Isolation. Transplantation 2005; 80 (6): 723.

  4. Molinari M, Al-Saif F, Ryan EA, Lakey JRT, Senior PA, Paty BW, Bigam D, Kneteman NM, Shapiro AMJ. Sirolimus-Induced Ulceration of the Small Bowel in Islet Transplant Recipients: Report of Two Cases. Am J Transpl 2005; 5: 1-6.

  5. Senior PA, Paty BW, Cockfield SM, Ryan EA, Shapiro AMJ. Case Report: Proteinuria Developing After Clinical Islet Transplantation Resolves with Sirolimus Withdrawal and Increased Tacrolimus Dosing. Am J Transpl 2005; 5: 2318-2323.

  6. Brennan DC, Shapiro AMJ. Thrombosis and Islet Transplantation – Letter to the Editor. Transplantation 2005; 79(12): 1773.

  7. Luo B, Nanji SA, Schur CD, Pawlick RL, Anderson CC, Shapiro AMJ. Robust Tolerance to Fully Allogeneic Islet Transplants Achieved by Chimerism with Minimal Conditioning. Transplantation 2005; 80: 370-377.

  8. Ryan EA, Paty BW, Senior PA, Bigam D, Al-Fadhli E, Kneteman NM, Lakey JRT, Shapiro AMJ. Five-Year Follow-Up After Clinical Islet Transplantation. Diabetes 2005; 54:2060-2069.

  9. Emamaullee J, Liston P, Korneluk RG, Shapiro AMJ, Elliott JF. XIAP Overexpression in Islet Beta-cells Enhances Engraftment and Minimizes Hypoxia-Reperfusion Injury. Am J Transpl 2005; 5: 1297-1305.

  10. Nanji SA and Shapiro AMJ. Advances in Islet Transplantation in Humans. Diabetes, Obesity and Metabolism 2005; 8 (1), 15-25.

  11. Matsumoto S, Okitsu T, Iwanaga Y, Noguchi H, Nagata H, Yonekawa Y, Yamada Y, Fududa K, Tsukiyama K, Suzuki H, Kawasaki Y, Shimodaira M, Matsuoka K, Shibata T, Kasai Y, Maekawa T, Shapiro AMJ, Tanaka K. Insulin Independence After Living Donor Distal Pancreatectomy and Islet Allo-Transplantation. The Lancet 2005; Published on-line April 19, 2005.

  12. Senior PA, Welsh RC, McDonald CG, Paty BW, Shapiro AMJ, Ryan EA. Coronary Artery Disease is Common in Non-Uremic, Asymptomatic Type 1 Diabetic Islet Transplant Candidates. Diabetes Care 2005; 28: 866-872.

  13. Shapiro AMJ, Lakey JRT, Paty BW, Senior PA, Bigam DL, Ryan EA. Strategic Opportunities in Clinical Islet Transplantation. Transplantation 2005; 79(10); 1304-1307.

  14. Ryan EA, Paty BW, Senior PA, Lakey JRT, Bigam D, Al-Fadhli E, Shapiro AMJ. b-Score: An Assessment of Beta-Cell Function After Islet Transplantation. Diabetes Care 2005; 28: 343-347.

  15. Shapiro AMJ, Gallant HL, Hao EG, Lakey JRT, McCready T, Rajotte RV, Yatscoff RW, Kneteman NM. The Portal Immunosuppressive Storm – Relevance to Islet Transplantation? Ther Drug Mon 2005; 27(1); 35-37.

2004

• 1. Shapiro AMJ, Ricordi C. Unraveling the Secrets of Single Donor success in Islet Transplantation. Am J Transpl 2004; 4: 295-8.

  2. Johnson JA, Kotovych M, Ryan EA, Shapiro AMJ. Reduced Fear of Hypoglycemia in Successful Islet Transplantation. Diabetes Care 2004; 27(2): 624-5.

  3. Tsujimura T, Kuroda Y, Churchill TA, Avila JG, Kin T, Shapiro AMJ, Lakey JRT. Short-Term Storage of the Ischemically Damaged Human Pancreas by the Two-Layer Method Prior to Islet Isolation. Cell Transplantation 2004; 13:67-73.

  4. Ryan EA, Shandro T, Green K, Paty BW, Bigam D, Shapiro AMJ, Vantyghem MC. Assessment of the Severity of Hypoglycemia and Glycemic Lability in Type 1 Diabetic Subjects Undergoing Islet Transplantation. Diabetes 2004; 53:955-962.

  5. Rose NL, Palcic MM, Shapiro AMJ, Lakey JRT. Endogenous Pancreatic Enzyme Activity levels Show No Significant Effect on Human Islet Isolation Yield. Cell Transplantation 2004; 13:153-160.

  6. Paty BW, Bonner-Weir S, Laughlin M, McEwan AJ, Shapiro AMJ. Towards Development Of Imaging Modalities For Islets After Transplantation: Insights From The National Institutes Of Health Workshop On Beta Cell Imaging. Transplantation 2004; 77(8):1133-1137.

  7. Street CN, Lakey JRT, Seeberger K, Helms L, Rajotte RV, Shapiro AMJ, Korbutt GS. Heterogenous Expression of Nestin in Human Pancreatic Tissue Precludes Its Use As An Islet Precursor Marker. J Endocrinology 2004; 180: 213-225.

  8. Bhargava R, Senior PA, Ackerman TE, Ryan EA, Paty BW, Lakey JRT, Shapiro AMJ. Prevalence of Hepatic Steatosis After Islet Transplantation and Its Relationship to Graft Function. Diabetes 2004; 53:1311-1317.

  9. Tsujimura T, Kuroda Y, Avila JG, Kin T, Oberholzer J, Shapiro AMJ, Lakey JRT. Influence of Pancreas Preservation on Human Islet Isolation Outcomes: Impact of the Two-Layer Method. Transplantation 2004; 78(1): 96-100.

  10. Brennan DC, Desai N, Shannon MB, Robertson H, O’Brien L, Polonsky KS, Shapiro AMJ. Portal Vein Thrombosis Complicating Islet Transplantation in a Recipient with the Factor V Leiden Mutation. Transplantation 2004; 78(1): 172-173.

  11. Street CN, Lakey JRT, Rajotte RV, Shapiro AMJ, Kieffer TJ, Lyon JG, Kin T, Korbutt GS. Enriched Human Pancreatic Ductal Cultures Obtained from Selective Death of Acinar Cells Express Pancreatic and Duodenal Homeobox Gene-1 Age-Dependently. Rev Diab Stud 2004; 1:66-79.

  12. Senior PA, Shapiro AMJ, Ackerman TE, Ryan EA, Paty BW, Bhargava R. Magnetic Resonance-Defined Perinephric Edema After Clinical Islet Transplantation: A Benign Finding Associated With Mild Renal Impairment. Transplantation 2004; 78(6): 945-948.

  13. McDonald CG, Ryan EA, Paty BW, Senior PA, Marshall SM, Lakey JRT, Shapiro AMJ. Cross-Sectional and Prospective Association Between Proinsulin Secretion and Graft Function After Clinical Islet Transplantation. Transplantation 2004; 78(6): 934-937.

  14. Ryan EA, Paty BW, Senior PA, Shapiro AMJ. Risks and Side Effects of Islet Transplantation. Curr Diab Rep 2004; 4:304-309.

  15. Nanji SA, Shapiro AMJ. Islet Transplantation in Patients with Diabetes Mellitus: Choice of Immunosuppression. BioDrugs 2004; 18(5):315-328.

  16. Burridge PW, Shapiro AMJ, Ryan EA, Lakey JRT. Clinical Islet Transplantation in The New Millennium. J of the Egypt Soc Endocrin Metab and Diab 2004; 35(1&2):5-10.

  17. Barnett MJ, McGhee-Wilson D, Shapiro AMJ, Lakey JRT. Variation in Human Islet Viability Based on Different Membrane Integrity Stains. Cell Transplantation 2004; 13: 481-488.

  18. Street CN, Lakey JRT, Shapiro AMJ, Imes S, Rajotte RV, Ryan EA, Lyon JG, Kin T, Avila J, Tsujimura T, Korbutt GS. Islet Graft Assessment in the Edmonton Protocol: Implications for predicting long term clinical outcome. Diabetes 2004; 43: 3107-3114.

  19. Bigam DL, Shapiro AMJ. Pancreatic Transplantation. Curr Treatment Options in Gastroenterol 2004; 7(5):329-341.

2003

• 1. Orive G, Hernandez RM, Gascon AR, Calafiore R, Chang TMS, De Vos P, Hortelano G, Hunkeler D, Lacik I, Shapiro AMJ, Pedraz JL. Cell Encapsulation: Promise and Progress. Nature Medicine 2003; 9(1):104-107.

  2. Shapiro AMJ, Lakey JRT. Islet Transplant – A Secure Future for Cellular Replacement Therapy in Diabetes. Pract Diab Intl 2003; 20(4):1-5.

  3. Hathout E, Lakey JRT, Shapiro AMJ. Islet Transplant: An Option for Childhood Diabetes? Arch Dis Child 2003; 88(7):591-594.

  4. Shapiro AMJ. Islet Transplants and Impact on Secondary Diabetic Complications: Does C-Peptide Protect the Kidney? J Am Soc Nephrol 2003; 14:2214-2216.

  5. Lakey JRT, Burridge PW, Shapiro AMJ. Technical Aspects of Islet Preparation and Transplantation. Transpl Int 2003; 16(9):613-632.

  6. Oberholzer J, Shapiro AMJ, Lakey JR, Ryan EA, Rajotte RV, Korbutt GS, Morel P, Kneteman NM. Current Status of Islet Cell Transplantation. Adv Surg 2003; 37:253-282.

  7. Owen RJT, Ryan EA, O’Kelly K, Lakey FRT, McCarthy M, Paty BW, Bigam D, Kneteman NM, Korbutt GS, Rajotte RV, Shapiro AMJ. Percutaneous Transhepatic Pancreatic Islet Cell Transplantation in Type I Diabetes: Radiological Aspects. Radiology 2003; 229(1).

  8. Shapiro AMJ, Hering BJ, Ricordi C. Edmonton’s Success Has Indeed Been Replicated Elsewhere. The Lancet 2003; 362:1242.

  9. Toso C, Morel P, Bucher P, Shapiro AMJ, Mathe Z, Demuylder S, Bosco D, Berney T, Philippe J and Oberholzer J. Insulin-independence after Conversion to Tacrolimus and Sirolimus Based Immunosuppression in Islet-Kidney Recipients. Transplantation 2003; 76(7): 1133-1134.

  10. Rose NL, Palcic MM, Shapiro AMJ, Lakey JRT. An Evaluation of the Activation of Endogenous Pancreatic Enzymes During Human Islet Isolations. Transplantation Proceedings 2003; 35: 2455-7.

  11. Rose NL, Palcic MM, Shapiro AMJ, Lakey JRT. An Evaluation of Endogenous Pancreatic Enzyme Levels Following Enzymatic Digestion. Transplantation Proceedings 2003; 35:2458-60.

  12. Tsujimura T, Kuroda Y, Avila JG, Kin T, Churchill TA, Shapiro AMJ, Lakey JRT. Resuscitation of the Ischemically Damaged Human Pancreas by the Two-Layer Method Prior to Islet Isolation. Transplantation Proceedings 2003; 35: 2461-2.

  13. Shapiro AMJ, Nanji SA, Lakey JRT. Clinical Islet Transplant – Current and Future Directions Towards Tolerance. Immunological Reviews 2003; 196: 219-36.

  14. Rafael E, Ryan EA, Senior P, McDonald C, Paty BW, Imes S, Shapiro AMJ, Olberholzer J, Lakey JRT. Changes in Liver Enzymes After Clinical Islet Transplantation. Transplantation 2003; 76: 1280-4.

2002

1. Shapiro AMJ, Suarez-Pinzon WL, Power R and Rabinovitch A. Combination Therapy with Low Dose Sirolimus and Tacrolimus is Synergistic in Preventing Spontaneous and Recurrent Autoimmune Diabetes in Non-Obese Diabetic Mice. Diabetologia 2002; 45:224-230.

2. Rabinovich A, Suarez-Pinzon WL, Shapiro AMJ, Rajotte RV and Power R. Combination Therapy with Sirolimus and Interleukin-2 Induces Long-Term Prevention of Spontaneous and Recurrent Autoimmune Diabetes in NOD Mice. Diabetes 2002; 51:638-645.

3. Lakey JRT, Tsujimura T, Shapiro AMJ and Kuroda Y. Human Pancreas Preservation Prior to Islet Isolation. Cell Preservation Technology 2002; 1(1): 81-87.

4. Lakey JR, Kneteman NM, Rajotte RV, Wu DC, Bigam D and Shapiro AMJ. Effect of core pancreas temperature during cadaveric procurement on human islet isolation and functional viability. Transplantation 2002; 73(7): 1106-10.

5. Ryan EA, Lakey JRT, Paty BW, Imes S, Korbutt GS, Kneteman NM, Bigam D, Rajotte RV and Shapiro AMJ. Successful Islet Transplantation: Continued Insulin Reserve Provides Long-Term Glycemic Control. Diabetes 2002; 51(7): 2148-57.

6. Casey JJ, Lakey JRT, Ryan EA, Paty BW, Owen R, O’Kelly K, Nanji S, Rajotte RV, Korbutt GS, Bigam D, Kneteman NN and Shapiro AMJ. Portal Venous Pressure Changes following Sequential Clinical Islet Transplantation. Transplantation 2002; 74(7): 913-915.

7. Shapiro AMJ , Ryan E, Rajotte R, Korbutt G, Kneteman N, Bigam D, Paty, B, Lakey JRT. Improved Function of Islet Grafts Under Steroid-Free Immunosuppression: Will Grafts Work Forever? Transplantation 2002; 73(9): 1528.

8. Burridge PW, Shapiro AMJ, Ryan EA, Lakey JRT. Future Trends in Clinical Islet Transplantation. Transplantation Proceedings 2002; 34(8):3347-8.

9. Paty BW, Ryan EA, Shapiro AMJ, Lakey JRT and Robertson RP. Intrahepatic Islet Transplantation in Type 1 Diabetic Patients Does Not Restore Hypoglycemic Hormonal Counterregulation or Symptom Recognition After Insulin Independence. Diabetes 2002; 51:3428-34.

10. Shapiro AMJ . Eighty Years After Insulin: Parallels with Modern Islet Transplantation. CMAJ 2002; 167(12):1398-1400.

11. Tsujimura, T, Kuroda Y, Kin T, Avila JG, Rajotte RV, Korbutt GS, Ryan EA, Shapiro AMJ, Lakey FRT. Human Islet Transplantation from Pancreases with Prolonged Cold Ischemia Using Additional Preservation by the Two-Layer (UW Solution/Perfluorochemical) Cold Storage Method. Transplantation 2002; 74(12):1687-91.

2001

1. Shapiro AMJ, Ryan EA and Lakey JRT. Islet Cell Transplantation. The Lancet Suppl 2001; 358: S21

2. Shapiro AMJ, Ryan E, Warnock G, Kneteman NM, Lakey J, Korbutt GS and Rajotte RV. Could fewer islet cells be transplanted in type 1 diabetes? Insulin independence should be the dominant force in islet transplantation. BMJ 2001; 322:861

3. Lakey JRT, Helms LMH, Kin T, Korbutt GS, Rajotte RV, Shapiro AMJ and Warnock GL. Serine-Protease Inhibition During Islet Isolation Increases Islet Yield from Human Pancreases with Prolonged Ischemia. Transplantation 2001; 72 (4): 565.

4. Ryan EA, Lakey JRT, Rajotte RV, Korbutt GS, Kin T, Imes S, Rabinovitch A, Elliott JF, Bigam D, Kneteman NM, Warnock GL, Larsen I and Shapiro AMJ. Clinical Outcomes and Insulin Secretion After Islet Transplantation with the Edmonton Protocol. Diabetes 2001; 50:710-719.

5. Ryan EA, Lakey JR, Shapiro AMJ. Clinical Results After Islet Transplantation. J Investig Med 2001; 49(6):559-562.

2000

• 1. Shapiro AMJ, Lakey JRT, Ryan EA, Korbutt GS, Toth E, Warnock GL, Kneteman NM and Rajotte RV. Islet Transplantation in Seven Patients with Type 1 Diabetes Mellitus Using a Glucocorticoid-Free Immunosuppressive Regimen. N Engl J Med 2000; 343(4): 230-8.

For more information contact the Clinical Islet Transplant Program in Edmonton:

Clinical Islet Transplant Program

2000 College Plaza

8215-112th Street Edmonton, Alberta, Canada T6G 2C8

780-407-1501(phone)

780-407-3850(fax)

Email: isletprogram@islet.ca